The televised game is a joint 50th anniversary celebration of the creation of the European Union and of United becoming the first English side to play in UEFA's European club competition.
The Commission's first "signing", David Beckham, has had to drop out through injury, but will be at the match to see his old club face up to an EU team boasting Liverpool players Steven Gerrard and Jamie Carragher, Barcelona captain Carles Puyol and many of the Italian World Cup-winning squad.
Three Brazilian stars - Ronaldinho, Ronaldo and Juninho - will play as "guests", because they are not strictly eligible for a "Europe XI" team.
An unusually popular European Commission press conference saw Manchester United legend Sir Bobby Charlton take the stage alongside the Europe XI coach, Italian World Cup winner Marcello Lippi.
And UEFA President Michel Platini was on hand to present Commission President Jose Manuel Barroso with a Europe XI team shirt - number 27, to reflect the number of EU member states.
Sir Bobby said he was proud of Manchester United and proud of the European Union, and the match was a "very significant" occasion.
He added: "The history of Manchester United is tied up with that of Europe.
"Some of the club's greatest moments have been played out on a European stage.
"I look forward to welcoming so many stars and friends to Old Trafford to celebrate these two significant anniversaries."
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edinburgh red, edinburgh (12/03/2007 at 14:18)
Jonathan Chin, Truly Asia (12/03/2007 at 14:20)
Loyal to the End (31 years, City Fans, 31 years), Nottingham, ex-Malaysia (12/03/2007 at 14:29)
Demo, Glossop (12/03/2007 at 14:30)
Demo, Glossop (12/03/2007 at 14:56)
Frank Old Trafford (12/03/2007 at 16:11)
Macca, Cornwall (12/03/2007 at 16:14)
stephen.woodland@gmail.com, Manchester (12/03/2007 at 16:25)
David (12/03/2007 at 16:57)
ronaldinho blew you out TWICE.
come on smoggies 1/4 final replay!
Sean, NYXMC (12/03/2007 at 17:23)
The full Europe XI squad
Goalkeepers: Oliver Kahn, Grégory Coupet, Iker Casillas.
Defenders: Paolo Maldini, Jamie Carragher, Eric Abidal, Lilan Thuram, Carles Puyol, Marco Materazzi, Fabio Grosso.
Midfielders: Gianluca Zambrotta, Steven Gerrard, Juninho Pernambucano, Florent Malouda, Luis Miguel, Gennaro Gattuso, Andrea Pirlo.
Strikers: Ronaldinho, Ronaldo, Alessandro Mancini, Zlatan Ibrahimovic, Henrik Larsson.
The surprise for me is Larsson, I thought he was going to be playing for MU...
Gerry, Glastonbury (12/03/2007 at 17:42)
Gerry, Glastonbury (12/03/2007 at 17:45)
Chris Kenway (12/03/2007 at 18:11)
I wonder who the genius was that scheduled it for now--instead of, perhaps, during the pre-season?
Demo, Glossop (12/03/2007 at 18:23)
Whaley Bridge Cynic, Whaley Bridge (12/03/2007 at 19:25)
Can any of you United muppets spell respect?
Loyal to the End (31 years, City Fans, 31 years), Nottingham, ex-Malaysia (13/03/2007 at 00:14)
Loyal to the End (31 years, City Fans, 31 years), Nottingham, ex-Malaysia (13/03/2007 at 00:16)
Birdy, Ireland (13/03/2007 at 08:53)
ocnorb, wythenshawe (13/03/2007 at 09:49)
Steve in Brussels (13/03/2007 at 09:56)
stu, london (13/03/2007 at 11:46)