Wanderers face La Liga's fourth-placed Atletico on Valentine's Day at the Reebok Stadium.
Spaniard Ivan Campo wants to play against his compatriots after being unavailable for the earlier rounds.
But Megson can only register three new players for the second half of the competition. And new signings Gary Cahill, Matty Taylor and Grzegorz Rasiak also come into the reckoning.
Icelandic international Gretar Steinsson is ineligible, having played for AZ Alkmaar.
Cahill certainly wants to make a European bow with Bolton.
"We have a strong squad," said the former Aston Villa defender after an impressive debut against Reading on Saturday. "We're in Europe and you don't get to that level without having quality in the team. That's what we've got and that's why I came here."
Cahill and Kevin Nolan were both man of the match contenders against the Royals.
And while his England prospects don't come any closer, Nolan is delighted by Wanderers' recent renaissance.
"People laughed when I said we could finish in the top half and sometimes I think I shouldn't have said it.
Defence
"But I really do think that, with the squad we've got, we can break into the top ten.
"You've seen the Bolton of old come back in the last three games.
"The defence was fantastic, putting their heads in where it hurts.
"Some of the heading and the last-ditch tackles were absolutely fantastic.
"Three clean sheets in a row is massive boost, but we've got the goals as well."
Nolan hailed the contribution of manager Megson.
"He got a lot of stick when he came," he said. "A lot of people were doubting him.
"But the players he's brought in and the way he's got us playing now has set the foundations.
"The last two weeks have been the best since he's been here.
"He's not only brought good players in but he's been able to work with the team more than before.
"When he first came we were playing game after game and he had to deal with that.
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"But he's started to lay down the foundations and you can see the benefits of that in the way we played at Reading."
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Cheer up, Gary!